7,11-methano-5h-cyclodeca[3,4]benz[1,2-b]oxete benzenepropanoic acid deriv.
Paclitaxel
CAS: 33069-62-4
Molecular Formula: C47H51NO14
7,11-methano-5h-cyclodeca[3,4]benz[1,2-b]oxete benzenepropanoic acid deriv. - Names and Identifiers
| Name | Paclitaxel |
| Synonyms | taxal taxol a PACLITAXEL Paclitaxel PACLITAXOL Paclitaxelx Paclitaxel HCL PACLITAXEL, TAXUS SPECIES Paclitaxel(natural crude) PACLITAXEL, TAXUS BREVIFOLIA N-BENZYL-BETA-PHENYLISOSERINE ESTER 7,11-methano-5h-cyclodeca[3,4]benz[1,2-b]oxete benzenepropanoic acid deriv. |
| CAS | 33069-62-4 |
| EINECS | 608-826-9 |
| InChI | InChI=1/C47H51NO14/c1-25-31(60-43(56)36(52)35(28-16-10-7-11-17-28)48-41(54)29-18-12-8-13-19-29)23-47(57)40(61-42(55)30-20-14-9-15-21-30)38-45(6,32(51)22-33-46(38,24-58-33)62-27(3)50)39(53)37(59-26(2)49)34(25)44(47,4)5/h7-21,31-33,35-38,40,51-52,57H,22-24H2,1-6H3,(H,48,54)/t31?,32-,33+,35?,36?,37+,38?,40?,45+,46-,47+/m0/s1 |
| InChIKey | RCINICONZNJXQF-MZXODVADSA-N |
7,11-methano-5h-cyclodeca[3,4]benz[1,2-b]oxete benzenepropanoic acid deriv. - Physico-chemical Properties
| Molecular Formula | C47H51NO14 |
| Molar Mass | 853.92 |
| Density | 0.200 |
| Melting Point | 213°C (dec.)(lit.) |
| Boling Point | 774.66°C (rough estimate) |
| Specific Rotation(α) | D20 -49° (methanol) |
| Flash Point | 9℃ |
| Water Solubility | 0.3mg/L(37 ºC) |
| Solubility | Insoluble in water, easily soluble in chloroform, acetone and other organic solvents. |
| Vapor Presure | 0mmHg at 25°C |
| Appearance | White crystalline powder |
| Color | white |
| Maximum wavelength(λmax) | 227nm(MeOH)(lit.) |
| Merck | 14,6982 |
| BRN | 1420457 |
| pKa | 11.90±0.20(Predicted) |
| Storage Condition | 2-8°C |
| Stability | Stable. Incompatible with strong oxidizing agents. Combustible. |
| Refractive Index | -49 ° (C=1, MeOH) |
| MDL | MFCD00869953 |
| Physical and Chemical Properties | Melting Point: 213-216°C |
| Use | Broad-spectrum antineoplastic plant medicine for the treatment of ovarian cancer, breast cancer and other diseases |
7,11-methano-5h-cyclodeca[3,4]benz[1,2-b]oxete benzenepropanoic acid deriv. - Risk and Safety
| Hazard Symbols | Xn - Harmful |
| Risk Codes | R37/38 - Irritating to respiratory system and skin. R41 - Risk of serious damage to eyes R42/43 - May cause sensitization by inhalation and skin contact. R62 - Possible risk of impaired fertility R68 - Possible risk of irreversible effects R40 - Limited evidence of a carcinogenic effect R48 - Danger of serious damage to health by prolonged exposure R20/21/22 - Harmful by inhalation, in contact with skin and if swallowed. R68/20/21/22 - |
| Safety Description | S22 - Do not breathe dust. S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection. S45 - In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.) |
| UN IDs | 1544 |
| WGK Germany | 3 |
| RTECS | DA8340700 |
| FLUKA BRAND F CODES | 10-21 |
| HS Code | 29329990 |
| Hazard Class | 6.1(b) |
| Packing Group | III |
| Toxicity | LD50 intraperitoneal in mouse: 128mg/kg |
7,11-methano-5h-cyclodeca[3,4]benz[1,2-b]oxete benzenepropanoic acid deriv. - Introduction
Open Data Unverified Data
Taxol is a monomeric diterpenoid extracted from the bark of Taxus, which is a complex secondary metabolite, it is also the only known drug that can promote the polymerization of microtubules and stabilize the polymerized microtubules. Isotopic tracing indicates that paclitaxel binds only to polymerized microtubules and does not react with unpolymerized tubulin dimers. After exposure to paclitaxel, cells accumulate a large number of microtubules in the cell. The accumulation of these microtubules interferes with the various functions of the cell, especially the cell division stops in the mitotic phase, blocks the normal division of cells. Through Ⅱ-Ⅲ clinical research, paclitaxel is mainly applied to ovarian cancer and breast cancer, and has certain curative effect on lung cancer, colorectal cancer, melanoma, head and neck cancer, lymphoma and brain tumor.
Last Update：2022-01-01 08:50:50
7,11-methano-5h-cyclodeca[3,4]benz[1,2-b]oxete benzenepropanoic acid deriv. - Indication
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The treatment of ovarian cancer and platinum-resistant ovarian cancer, breast cancer has a good effect on the treatment of prostate cancer, head and neck cancer, esophageal cancer, germ cell tumors, endometrial cancer, lymphoma, bladder cancer, upper gastrointestinal cancer, small cell and non-small cell lung cancer have good prospects.
Last Update：2022-01-01 08:50:51
7,11-methano-5h-cyclodeca[3,4]benz[1,2-b]oxete benzenepropanoic acid deriv. - Nature
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The appearance was white crystalline or amorphous powder. It was first isolated from Taxus brevis by American chemist Wall et al. In1967. It can bind to tubulin and promote tubulin polymerization to assemble into microtubule dimer, thus inhibiting cell microtubule depolymerization and preventing cell rapid reproduction. Clinical trials have shown that paclitaxel has a significant effect in the treatment of metastatic ovarian cancer and breast cancer, and also has a certain effect in the treatment of small cell and non-small cell lung cancer, cervical cancer, anti-chemotherapy leukemia and so on. Taxol only exists in the genus Taxus, a total of 11 species, China has 4 species and 1 variety, they are TAXUS yunnanensis, Tibet TAXUS (also known as Himalaya), taxus chinensis, taxus chinensis var. Mairei (also known as Taxus chinensis). In addition to Taxol, these plants also contain a variety of other taxane diterpenes.
Last Update：2024-01-02 23:10:35
7,11-methano-5h-cyclodeca[3,4]benz[1,2-b]oxete benzenepropanoic acid deriv. - Preparation Method
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There are three methods for producing paclitaxel. The first is a synthetic method, the second is a bacterial culture method, and the third is a direct separation from Taxus. Although the first two methods have achieved significant research results, they have not achieved industrialization. At present, the separation from natural or cultivated Taxus is still the main way to produce Taxol. The separation process is generally methanol or ethanol leaching (or ultra-off boundary extraction) a hexane de-esterification of dichloromethane or chloroform extraction of crude extract a multiple silica gel column chromatography preparative HPLC, TLC, HSCCC was purified and recrystallized to give the product. Since it is difficult to separate paclitaxel from another derivative Cephal omanmne by silica gel column chromatography and the product yield is low, Kingston et al. used 03 to selectively oxidize Olefinic Bonds in Cepha-lomannine molecules, and then purified paclitaxel by silica gel column chromatography. In recent years, great progress has been made in the decolorization and separation of crude extracts of Taxus chinensis by using polymer resin, which overcomes the problems of low yield, small yield, high cost and long growth cycle of the original separation methods.
Last Update：2022-01-01 11:15:24
7,11-methano-5h-cyclodeca[3,4]benz[1,2-b]oxete benzenepropanoic acid deriv. - Introduction
Pharmacological effects: Paclitaxel is mainly suitable for ovarian cancer and breast cancer. It also has a certain effect on lung cancer, colorectal cancer, melanoma, head and neck cancer, lymphoma, and brain tumor.
Last Update：2022-10-16 17:24:39
7,11-methano-5h-cyclodeca[3,4]benz[1,2-b]oxete benzenepropanoic acid deriv. - Small History
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- American chemists M.C. Wani and Monre E. Wall) for the first time from a large forest in the western United States called Pacific Yew (Pacific Yew) in the bark and wood isolated from the crude extract of paclitaxel. In the screening experiment of Taxus chinensis, Wani and Wall found that the crude extract of paclitaxel had high activity on rat tumor cells cultured in vitro, and began to isolate this active ingredient. Because of the extremely low content of the active ingredient in plants, it was not until 1971 that they shared with the professor of chemistry at Duke University, amkfall (andrt. Et al. The chemical structure of the active ingredient, a tetracyclic diterpene compound, was determined by x-ray analysis and named taxol.
- paclitaxel was found in Taxus chinensis in 1971, and its anticancer mechanism was found to be unique.
- in 1992, the US government transferred the patent to Bristol-Myers Squibb, and paclitaxel was launched.
- The World Anti-Cancer Drug Sales of paclitaxel in 1994.
- Paclitaxel sales hit 10 billion in 2000 (after no further increase in the supply of raw materials)
- in 2002, the central government issued a document prohibiting the felling of wild Taxus species and encouraging artificial cultivation.
- Bristol-Myers Squibb patent expired in 2004, and more pharmaceutical factories around the world involved in the production of paclitaxel.
- in 2004, Huayuan began to operate the Taxus project and set up a Taxol refining and processing plant directly under the plan, with the target of being the largest TAXUS base in China and even Asia.
- in 2005, the central government issued a new document, the national census of TAXUS resources, encourage planting.
- in 2005, this project accepted investment from individual investors.
Last Update：2022-01-01 08:50:52
7,11-methano-5h-cyclodeca[3,4]benz[1,2-b]oxete benzenepropanoic acid deriv. - Use
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can be used for the treatment of metastatic ovarian cancer and breast cancer; Also for the treatment of small cell and non-cell lung cancer, cervical cancer, anti-chemotherapy leukemia and so on.
Last Update：2022-01-01 11:15:25
7,11-methano-5h-cyclodeca[3,4]benz[1,2-b]oxete benzenepropanoic acid deriv. - Efficient separation and purification of Taxol
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- This includes: a. Extraction to obtain an extract containing Taxol using TAXUS as a raw material; B. Removal of colloid to remove colloid impurities in the extract; And c. Separation and purification.
- the production process of paclitaxel is as follows: the bark of Taxus chinensis is crushed (the finer the better),85% ~ 95% alcohol (what is the ratio of material to liquid?) 35-55 ° C hot reflux extraction three times (how much time is needed each time?), 50-70 ℃ vacuum decompression concentration to heat specific gravity 1.1~1.2g/ml, chloroform extraction, extract concentration into paste, paclitaxel content 1% chloroform paste, the paclitaxel content of 1% chloroform paste and chloroform dissolved completely, and silica gel stirred evenly, cool dry, sieved, filled into the column, chloroform-methanol gradient elution, TLC detection, segment combined concentration, the semi-finished product with paclitaxel content of 5-8% was prepared. The semi-finished product with paclitaxel content of 5-8% was completely dissolved by adding acetone, mixed with silica gel, dried, sieved, and filled into the chromatography column, acetone-petroleum ether gradient elution, TLC detection, combined and concentrated, paclitaxel content of 20 ~ 25% semi-finished products, with acetone-petroleum ether system crystallization 3~4 times, Suction filtration, 50 ℃ vacuum drying under reduced pressure, the semi-finished product with paclitaxel content of 75-80% was obtained, and the product with paclitaxel content ≥ 99.5% was obtained by 16Mpa pressure chromatography, TLC detection, fractional merging and concentration, acetone-petroleum ether crystallization of target concentrate, Suction filtration and drying;
- the process of removing the colloid is as follows: the colloid is removed by high-pressure silica gel column chromatography, and the taxane compound is separated into paclitaxel, cephalomannine and 7-taxol.
Last Update：2022-01-01 08:50:52
7,11-methano-5h-cyclodeca[3,4]benz[1,2-b]oxete benzenepropanoic acid deriv. - Docetaxel
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docetaxel is a potent inhibitor of bone marrow activity derived from the yew tree. Blood cell and platelet counts are monitored during treatment. The mechanism of action of the drug is similar to that of paclitaxel, which inhibits the depolymerization of microtubules and inhibits cell division. Treatment of advanced or metastatic breast cancer and non-small cell lung cancer by intravenous infusion.
Last Update：2022-01-01 08:50:53
7,11-methano-5h-cyclodeca[3,4]benz[1,2-b]oxete benzenepropanoic acid deriv. - International Market Analysis
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- so far, there are only two kinds of paclitaxel raw materials on the international market: one is from the bark of various Taxus species; the other is to extract the "10-berry red alkali" from the branches of European ornamental Taxus, and then semi-synthesized, that is, docetaxel (docetaxel), its structure is very similar to the natural extraction of paclitaxel. These two kinds of raw materials are the best-selling raw material products in the international pharmaceutical market, and have been in short supply for a long time. According to the estimation of relevant departments, the sales volume ratio of paclitaxel and docetaxel is about 10:1.
- in the United States, Paclitaxel Injection (trade name: Paclitaxel) is mainly exclusively produced by Bristol-Myers Squibb, and there are about two or three pharmaceutical companies producing Paclitaxel raw materials and preparations, however, the market is basically a monopoly of Bristol-Myers Squibb, and the total sales of paclitaxel preparations produced by several other companies are only a fraction of Bristol-Myers Squibb. While the docetaxel drug substance and preparation are mainly produced by the French Planck Company and several companies such as the United Kingdom, Italy and so on, Planck has the largest output.
- paclitaxel is the preferred anti-tumor drug in hospitals all over the world. In recent years, the world's tumor Incidence Rate than 10 years ago has nearly doubled, lung cancer, breast cancer and ovarian cancer and other malignant tumors also showed a multiple trend, these cancer patients are the main users of paclitaxel. Overall, sales of paclitaxel will only rise, not decline, until no new plant-based anti-cancer drug can take its place.
- According to the latest figures released World Health Organization (WHO), the United States has become the world's first cancer-prone country, with 1.4 million new cancer cases each year, accounting for about 14% of the global cancer patients that year. The United States is still the largest consumer of paclitaxel. With the increase in cancer Incidence Rate in the United States, paclitaxel is the main drug against advanced malignant tumors, and it is believed that its sales volume will continue to maintain a rapid upward trend. The United States and other developed countries have already summed up a set of optimal compatibility schemes for paclitaxel and other anticancer drugs in the course of clinical use for more than ten years, the medical sector has been inseparable from the "backbone" of paclitaxel, therefore, the demand for paclitaxel raw material will also be rising.
Last Update：2022-01-01 08:50:54
7,11-methano-5h-cyclodeca[3,4]benz[1,2-b]oxete benzenepropanoic acid deriv. - Domestic situation
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Jiangsu Red Bean Group has invested tens of millions of yuan three or four years ago to build the largest Taxus chinensis fast-growing forest base in east China, with a total area of 7400 mu, and received high praise from the relevant departments of the United Nations and the leaders of the domestic Forestry Department. It is expected that the Red Bean Group will start production of paclitaxel raw material and injection this year, and design an annual output of 6 million paclitaxel injections. The project has passed the acceptance of the National Drug Inspection Bureau and the provincial and municipal drug administration.
In addition, China's Northeast forest area and some mountainous areas in Yunnan and Guizhou provinces are also vigorously developing TAXUS fast-growing forest cultivation techniques. In the next 3 to 5 years, these places are expected to form new TAXUS forest areas, so as to provide a valuable new source of paclitaxel raw material in China. Hunan, Hubei and other southern mountainous provinces are also building TAXUS cultivation bases, as one of the measures to help local farmers out of poverty and become rich. According to this development trend, the annual production of paclitaxel in China is expected to exceed 100 in the next few years, thus becoming the world's major producer of paclitaxel raw materials and preparations.
Last Update：2022-01-01 08:50:54
7,11-methano-5h-cyclodeca[3,4]benz[1,2-b]oxete benzenepropanoic acid deriv. - Precautions for use of injection
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note
- hematological toxicity: as the main factor limiting the dose increase, G-CSF should be used when the white blood cell is less than 1500/mm3, and the blood component should be transfused when the platelet is less than 30,000/mm3.
- allergic reaction: in addition to pretreatment, if only mild symptoms such as facial flushing, skin reaction, heart rate slightly faster, slightly lower blood pressure, it is not necessary to stop the drug, can slow down the drop rate. However, if there are serious reactions such as low blood pressure, angioedema, Dyspnea, General measles, should be discontinued and given appropriate treatment. Patients with severe allergies should not be treated with paclitaxel again.
- nervous system: numbness of fingers and toes was the most common. Significant sensory and motor disturbances and reduced tendon reflexes occur in about 4% of patients, particularly at high doses. There have been individual reports of grand mal seizures during instillation.
- Cardiovascular: transient tachycardia and hypotension are common and generally do not require treatment. However, in the first hour of infusion should be closely observed, in addition to serious conduction block patients do not have to be observed every hour.
- joints and muscles: about half of the patients will feel the joints 2-3 days after administration and Myalgia, which is related to the dosage used. Usually within a few days of recovery. Muscle pain is aggravated in G-CSF of patients given.
- hepatobiliary system: since most of paclitaxel is excreted from bile, patients with hepatobiliary diseases should be carefully observed. In thousands of cases, about 8% of patients had elevated bilirubin, 23% had elevated alkaline phosphatase, and 18% had elevated aspartate aminotransferase. However, there is no information that paclitaxel has serious damage to liver function.
- Other: gastrointestinal reactions are common but generally not severe, a few may have Diarrhea and mucositis. Mild alopecia is also common.
adverse reactions
- allergic reaction: the incidence rate was 39%, of which the incidence rate of severe allergic reaction was 2%. The majority of type 1 allergies, manifested as bronchial spastic Dyspnea, urticaria and hypotension. Almost all reactions occurred during the first 10 minutes after dosing.
- Myelosuppression: The main dose-limiting toxicity, manifested as neutropenia, thrombocytopenia rare, generally occurred after 8 to 10 days. The incidence of severe neutrophils was 47%, and the incidence of severe thrombocytopenia was 5%. Anemia is more common.
- neurotoxicity: the incidence of peripheral neuropathy was 62%, the most common manifestations were mild numbness and paresthesia, and the incidence of severe neurotoxicity was 6%.
- cardiovascular toxicity: there may be hypotension and asymptomatic short-term bradycardia. Muscle and joint pain: the incidence was 55%, occurred in the joints of the limbs, the incidence and severity were dose-dependent.
- gastrointestinal reaction: the incidence of Nausea, Vomit, Diarrhea and mucositis was 59%,43% and 39% respectively, generally mild and moderate.
- hepatotoxicity: ALT, elevated AST and AKP.
- alopecia: The incidence rate was 80%.
- Local reactions: inflammation in the local area of intravenous infusion of the drug and extravasation of the drug.
Last Update：2022-01-01 08:50:55
7,11-methano-5h-cyclodeca[3,4]benz[1,2-b]oxete benzenepropanoic acid deriv. - Chemical properties
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needle-like crystals or amorphous powders were precipitated from methanol. Melting Point 213~216 °c (decomposition). [Α] D20-49 ° (methanol). UV absorption maximum (methanol):227,273nm(& epsilon;29800,1700).
Last Update：2022-01-01 08:50:56
7,11-methano-5h-cyclodeca[3,4]benz[1,2-b]oxete benzenepropanoic acid deriv. - Production method
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- the content of natural products isolated and purified from the bark of yew, root of wood, leaves, shoots and seedlings was the highest in bark. TAXUS in the Chinese botanical record of the scientific name for the yew, plant classification into gymnosperm, pine, Taxus, TAXUS family, TAXUS genus. Taxaceae contains 5 genera and 23 species, and there are 4 genera and 12 species and 1 variety in China, which are Taxus wallichinanaZucc, taxus chinensis vat.Mairei) and Taxus cuspidata. Distributed in Tibet, Yunnan, Guizhou, Sichuan, Guangxi, Guangdong, Hunan, Hubei, Jiangxi, Fujian, Zhejiang, Anhui, Henan, Shanxi, Shaanxi, Gansu and Jilin mountainous areas.
- extracted from the bark or leaves of TAXUS plants. The bark or leaves of Taxus chinensis were dried in the shade, ground, and extracted with 95% ethanol. The extract was concentrated to dryness and the residue was stirred with water and dichloromethane. The organic layer was separated by standing and the aqueous layer was extracted several times with dichloromethane. The extracts and the organic layer were combined and concentrated to dryness. The residue was dissolved in ethyl acetate-methanol (3:1) and mixed with fresh celite, and the solvent was removed under reduced pressure. The remaining powder was subjected to flash column chromatography, eluting with hexane followed by dichloromethane. The latter was collected and the methylene chloride was distilled off under reduced pressure. The residue was dissolved in ethyl acetate and chromatographed on 70 sets (3-4 per set) of medium pressure flash chromatography columns filled with silica gel, eluting with hexane-acetone of different proportions. Fractions containing the product were collected and concentrated. The residue was purified by a medium pressure flash chromatography column packed with silica gel, eluting with different ratios of methanol-dichloromethane. The product-containing fractions were collected and concentrated under reduced pressure. The residue was separated by preparative high performance liquid chromatography, and the obtained product was recrystallized twice with aqueous methanol to obtain pure paclitaxel. Yield: bark 0.028%, leaves 0.0088%. Yield: bark 93.3%, leaves 88%. Melting Point: 212~214 ℃,[α] D20-49 degrees (1%, chloroform).
- the bark of Taxus chinensis was crushed, dried in the shade, and soaked with 1% citric acid aqueous solution for 24h before percolation. The permeate was extracted with dichloromethane, and the extract was dried, concentrated and vacuum-dried. Column chromatography was performed twice on silica gel, and the collected effluent contained paclitaxel and cephalomannine. Based on the difference of their side chain structure, they were dissolved in carbon tetrachloride with bromine, and only the three of the three of the bromine, and then through silica gel column chromatography, can only contain the effluent of paclitaxel, after treatment, the Taxol quality product was obtained, the content was 99.19%, the yield was 4.5 × 10-5, and the recovery was 70%.
Last Update：2022-01-01 08:50:56
7,11-methano-5h-cyclodeca[3,4]benz[1,2-b]oxete benzenepropanoic acid deriv. - Identification method
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a. Infrared absorption: The main absorption band in the infrared spectrum is consistent with the control.
B. HPLC identification: in the content detection, the retention time of the main peak in the chromatogram prepared by the detection is consistent with the retention time of the main peak in the standard preparation chromatogram.
Purity: 99-100%, based on dry product without water and solvent.
Related Substances: total related substances ≤ 2.0%
Organic volatile impurities: meet the requirements of the United States Pharmacopoeia (USP) and Chinese Pharmacopoeia (CP) organic volatile impurities.
Specific rotation:[α]20 D =-49.0 ° ~ 55.0 °(10mg/mL methanol solution), based on dry product without water and solvent.
Moisture: ≤ 4.0%
Residue: ≤ 0.2%.
Last Update：2022-01-01 08:50:57
7,11-methano-5h-cyclodeca[3,4]benz[1,2-b]oxete benzenepropanoic acid deriv. - Separation method
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step
This includes a. Extraction to obtain an extract containing Taxol using TAXUS as a raw material; B. Removal of pectin and removal of colloid impurities in the extract; And c. Separation and purification.
Process characteristics
The bark of Taxus chinensis is crushed (the finer the better),85% ~ 95% alcohol (what is the ratio of material to liquid?) 35-55 ° C hot reflux extraction three times (how much time is needed each time?), 50-70 ℃ vacuum decompression concentration to heat specific gravity 1.1~1.2g/ml, chloroform extraction, extract concentration into paste, paclitaxel content 1% chloroform paste, the paclitaxel content of 1% chloroform paste and chloroform dissolved completely, and silica gel stirred evenly, cool dry, sieved, filled into the column, chloroform-methanol gradient elution, TLC detection, segment combined concentration, the semi-finished product with paclitaxel content of 5-8% was prepared. The semi-finished product with paclitaxel content of 5-8% was completely dissolved by adding acetone, mixed with silica gel, dried, sieved, and filled into the chromatography column, acetone-petroleum ether gradient elution, TLC detection, combined and concentrated, paclitaxel content of 20 ~ 25% semi-finished products, with acetone-petroleum ether system crystallization 3~4 times, Suction filtration, 50 ℃ vacuum drying under reduced pressure, the semi-finished product with paclitaxel content of 75-80% was obtained. The product with paclitaxel content ≥ 99.5% was obtained by 16Mpa pressure chromatography, TLC detection, fractional merging and concentration.
the process of removing colloid
The high-pressure silica gel column chromatography removes the colloid, and simultaneously, the taxane compound is separated into paclitaxel, cephalomannine, and 7-epipaclitaxel 3 fractions.
side effects of slow release chemotherapy
- Clinical studies have shown that taking malt selenium to patients while using paclitaxel can help reduce cardiovascular toxicity and gastrointestinal reactions, and some cancer cells are less sensitive to paclitaxel, at this time, malt selenium can help control the killing of such cancer cells, and on the other hand, it has been proved to improve the activity of white blood cells.
- when taking chemotherapy and chemotherapy drugs at the same time, it can effectively enhance the activity of lymphokine il-12 and so on, enhance immunity, stimulate bone marrow and restore hematopoietic function of bone marrow, in addition, it can enhance the tolerance of cells to the toxicity of chemical drugs, reduce side effects and enhance the effect of chemotherapy.
- Chinese Journal of Oncology published an article by Jiao Yulian et al. "Ginsenoside Rh2 enhances paclitaxel-induced apoptosis of Hela cells" in 2006. The article pointed out: ginsenoside Rh2 can synergistically enhance the inhibitory effect of paclitaxel on the proliferation and apoptosis of H ela cells.
Last Update：2022-01-01 08:50:58
7,11-methano-5h-cyclodeca[3,4]benz[1,2-b]oxete benzenepropanoic acid deriv. - Method for Determination of content
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chromatographic conditions and system suitability test using eighteen alkyl silane bonded silica gel as filler; Methanol-water-acetonitrile (23:41:36) as mobile phase, the detection wavelength was 227nm. The system applicable solution 10 & micro;l under related substances shall be injected into the liquid chromatograph, and the separation degree of paclitaxel peak from paclitaxel impurity A peak and impurity B peak shall be greater than 1.0.
standard sample of paclitaxel (manufactured by Guizhou di-da), about 12mg, precision weighing, add 100ml measuring flask, add acetonitrile to dissolve and dilute to the scale, shake, 10 & micro;l was injected into the liquid chromatograph for precise measurement, and the chromatogram was recorded. According to the external standard method to calculate the peak area, that is.
This product is natural extraction or semi-synthetic preparation. This product is (2S,5R,7S,10R,13s)-10, 20-bis (acetyloxy)-2-benzoyloxy -1, 7-dihydroxy-9-oxo-5, 20-epoxytaxane -11-ene -13-YL (3S)-3-benzoylamino-3-phenyl-d-lactate. The content of C47H51NO14 shall be 98.0% ~ 102.0% calculated as dry product.
trait
- This product is white or off-white crystalline powder.
- This product is dissolved in methanol, ethanol or chloroform, slightly soluble in ether, almost insoluble in water.
- the specific rotation of this product is accurately weighed, dissolved in methanol and quantitatively diluted to prepare a solution containing 10mg per 1ml, which is determined according to law (Appendix VI E), the specific rotation was -49.0 ° to -55.0 °.
identification
- in the chromatogram under the content determination item, the main peak of the test solution should be consistent with the retention time of the main peak of the reference solution. The infrared absorption spectrum of
- paclitaxel should be consistent with that of the control (Spectrum set 875).
examination
- the clarity and color of the solution take this product 0.1g, add methanol 10ml to dissolve, the solution should be clear and colorless.
- Related substances take this product and add acetonitrile to make a solution containing about 0.5mg per 1ml as a test solution, add acetonitrile diluted to each 1ml containing about 2.5 & micro;g, 0.5 & micro;g of the solution, as control solution (1) and control solution (2); Take paclitaxel, paclitaxel impurity A (taxotraline) and paclitaxel impurity B(7-Table 10-deacetylpaclitaxel) control appropriate amounts, dissolved in acetonitrile and diluted to prepare paclitaxel containing about 0.5mg per 1ml, paclitaxel impurity A and paclitaxel impurity B were both 2.5 & micro;g solutions, as the system suitability solution. As determined by high performance liquid chromatography (Appendix V D), silica gel bonded with eighteen alkyl silanes is filled with
Last Update：2022-01-01 08:50:58
7,11-methano-5h-cyclodeca[3,4]benz[1,2-b]oxete benzenepropanoic acid deriv. - Pharmacological action
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- microtubules are a component of eukaryotic cells that are formed from microtubule dimers composed of two similar polypeptide (a and p) subunits. Under normal conditions, there is a dynamic equilibrium between microtubules and tubulin dimers. Paclitaxel can make the loss of this dynamic balance between the two, induce and promote tubulin polymerization, prevent depolymerization, and stabilize microtubules. These effects result in the inability of the cells to form spindles and spindles during mitosis, inhibit cell division and proliferation, and thereby exert an antitumor effect.
- in vitro studies have shown that paclitaxel binds reversibly to microtubules, especially to the P subunit of N-terminal tubulin, this action reduces the concentration of tubulin required for polymerization, shifts the dynamic equilibrium towards microtubule assembly, and increases the rate and yield of microtubule polymerization. The microtubules induced by paclitaxel were shorter and were about ten times more flexible than the microtubules normally formed without paclitaxel. Paclitaxel binds to microtubules in a 1:1 ratio, indicating that the drug has only one binding site on the microtubules. In addition, paclitaxel inhibits the normal dynamic regeneration of the micro-network necessary for mitosis, prevents the formation of normal mitotic spindles, causes the breakage of chromosomes, and inhibits the replication of cells. 1.5~5.(Vg/L paclitaxel was incubated with CHO and A2780 ovarian cancer cell lines for 24 h,99% of the cells died, and 57% of the cells entered the human Division phase, and extensive nuclear damage occurred. Paclitaxel alters the mitotic process of cells, increasing mitotic duration from 0-5 h to 15 h, and inhibits cytoplasmic division, resulting in the formation of multinucleated cells. These multinucleate cells continue to revert to the & phase and then attempt to undergo mitosis again, but there are no resting cells in mitosis. Micronuclei are also observed in many cells. Inhibition of spindle formation appears to be associated with this abnormal mitosis. In vitro, low concentration of paclitaxel (less than 8. 5 pg/L) blocked the transition from the middle to the late phase of the cell cycle and prevented cell proliferation without increasing the amount of microtubule polymers or forming microtubule spindles. Paclitaxel inhibits cell proliferation to a degree that parallels that of blocking metaphase of cell division. Higher concentrations of paclitaxel (greater than 8.5 fig/L) increased the amount of microtubule multimers at 282 pg/L, 500% higher than control levels, and resulted in the formation of large spindle microtubules. Leukemic cells were incubated with paclitaxel at a concentration of 85 pg/L or greater for 24 h to shrink 40% of the cells and concentrate the chromosomes.
Last Update：2022-01-01 08:50:59
7,11-methano-5h-cyclodeca[3,4]benz[1,2-b]oxete benzenepropanoic acid deriv. - Reference Information
| overview | paclitaxel is a monomeric diterpenoid extracted from the bark of the natural plant Taxus genus. it is a complex secondary metabolite and the only drug known so far that can promote microtubule polymerization and stabilize polymerized microtubules. Isotope tracing showed that paclitaxel only bound to the polymerized microtubules and did not react with the unpolymerized tubulin dimer. After contacting paclitaxel, cells will accumulate a large number of microtubules in the cell. The accumulation of these microtubules interferes with various functions of the cell, especially the cell division stops in the mitotic phase and blocks the normal division of the cell. Through Ⅱ-Ⅲ clinical research, paclitaxel is mainly suitable for ovarian cancer and breast cancer, and has certain curative effect on lung cancer, colorectal cancer, melanoma, head and neck cancer, lymphoma and brain tumor. |
| indication | treatment of ovarian cancer and platinum-resistant refractory ovarian cancer and breast cancer has a good effect, and has a good prospect for the treatment of prostate cancer, head and neck cancer, esophageal cancer, germ cell tumor, endometrial cancer, lymphoma, bladder cancer, upper gastrointestinal cancer, small cell and non-small cell lung cancer. |
| small history | In 1963, American chemists M.C. Wani and Monre E. Wall first isolated a crude extract of paclitaxel from the bark and wood of a Pacific fir (Pacific Yew) growing in the great forest of western United States. In the screening experiment of Taxus chinensis, Wani and Wall found that the crude extract of paclitaxel had high activity on in vitro cultured mouse tumor cells, and began to isolate this active ingredient. Due to the extremely low content of the active ingredient in plants, it was not until 1971 that they cooperated with Andre T. McPhail, a professor of chemistry at Duke University, to determine the active ingredient through x-ray analysis The chemical structure-a tetricyclic diterpene compound, and named it taxol. Taxol was discovered in Taxus chinensis in 1971 and found a unique anti-cancer mechanism. In 1992, the U.S. government transferred the patent to Squibb, and paclitaxel was introduced. In 1994, paclitaxel created the world's global sales champion of anti-cancer drugs. In 2000, the sales volume of paclitaxel reached 10 billion (the supply of raw materials did not increase further). In 2002, the central government issued a document prohibiting the cutting of wild yew and encouraging artificial planting. In 2004, Squibb's patent expired, and more pharmaceutical companies around the world were involved in the production of paclitaxel. In 2004, Huayuan began to operate the yew project and set up a directly affiliated paclitaxel refining and processing plant, with the goal of becoming the largest yew base in China and even Asia. In 2005, the central government issued a document again to conduct a national census of yew resources and encourage planting. In 2005, this project accepted investment from individual investors. |
| method for efficient separation and purification of paclitaxel | it includes a, extraction, using yew as raw material to obtain an extract containing paclitaxel; B, remove colloid, remove colloid impurities in the extract; C. separation and purification. It is characterized in that the production process of paclitaxel is as follows: yew bark is crushed (the finer the better),85% ~ 95% alcohol (what is the ratio of solid to liquid?)35-55 ℃ hot reflux leaching three times (how long does it take each time?),50-70 ℃ vacuum decompression concentration to thermal specific gravity of 1.1~1.2 g/ml, chloroform extraction, extraction liquid concentration into paste, to obtain paclitaxel content of 1% chloroform paste, paclitaxel content of 1% chloroform paste with chloroform dissolved completely, add silica gel to stir evenly, cool and dry, sieve, fill into chromatography column, chloroform-methanol gradient elution, TLC detection, segmented concentration, to obtain paclitaxel content of 5 ~ 8% semi-finished products, the semi-finished product with paclitaxel content of 5-8% is completely dissolved with acetone, silica gel is added and stirred evenly, cooled and dried, sieved, and filled into a chromatography column. Acetone-petroleum ether gradient elution, TLC detection, segmented combination and concentration are carried out to obtain semi-finished product with paclitaxel content of 20-25%, crystallized with acetone-petroleum ether system for 3-4 times, filtered by suction, vacuum decompression drying at 50 ℃ to obtain semi-finished product with paclitaxel content of 75-80%, which is, TLC detection, segmented consolidation and concentration, target segment concentrate acetone-petroleum ether crystallization, suction filtration, drying, to obtain taxol content ≥ 99.5% finished products; The process of removing colloid is: high pressure silica gel chromatography column chromatography to remove colloid, and at the same time separate taxane compounds into paclitaxel, harringtonine, 7-table paclitaxel 3 parts. |
| docetaxel | docetaxel is a very strong bone marrow activity inhibitor drug extracted from yew tree. blood cell and platelet count must be monitored during treatment. The mechanism of action of the drug is similar to that of paclitaxel, that is, it inhibits the depolymerization of microtubules and inhibits cell division. Treatment of advanced or metastatic breast and non-small cell lung cancer by intravenous infusion. |
| international market analysis | so far, there are only two kinds of paclitaxel raw materials in the international market: one is from the bark of various yew; The other is to extract "10-berylatin" from European ornamental yew branches and then semi-synthesize it, the structure of docetaxel (docetaxel) is very similar to that of naturally extracted paclitaxel. These two APIs are best-selling APIs in the international pharmaceutical market, and have been in short supply for a long time. According to estimates by relevant departments, the sales ratio of paclitaxel APIs to docetaxel APIs is about 10:1. In the United States, paclitaxel injection (trade name: Paclitaxel) is mainly produced exclusively by Bristol-Myers Squibb. Currently, about two or three pharmaceutical companies produce paclitaxel raw materials and preparations, but the market is basically monopolized by Bristol-Myers Squibb. The total sales of paclitaxel preparations produced by several other companies are only a fraction of Bristol-Myers Squibb. Docetaxel APIs and preparations are mainly produced by France's Planck company, Britain, Italy and other companies, with Planck's largest output. Paclitaxel is the preferred anti-tumor drug in hospitals all over the world. In recent years, the incidence of tumors in various countries in the world has nearly doubled compared with 10 years ago. Malignant tumors such as lung cancer, breast cancer and ovarian cancer are also showing a frequent trend. These cancer patients are the main users of paclitaxel. On the whole, sales of paclitaxel will only rise, not fall, until no new plant-based anti-cancer drug can take its place. According to the latest figures released by the World Health Organization, the United States has now become the world's number one cancer-prone country, with 1.4 million new cancer cases each year, accounting for about 14% of the world's cancer patients that year. The United States is still the largest consumer of paclitaxel. With the increasing incidence of cancer in the United States, paclitaxel, as the main drug against advanced malignant tumors, can be believed that its sales will continue to maintain a rapid upward trend. The United States and other developed countries have already summarized a set of optimal compatibility schemes for paclitaxel and other anti-cancer drugs in the course of clinical use for more than ten years. Its medical department is inseparable from the "backbone" of paclitaxel. Therefore, the demand for paclitaxel raw materials will also rise. |
| domestic situation | Jiangsu hongdou group has invested tens of millions of yuan to build the largest fast-growing yew forest base in east China three or four years ago, with a total area of 7400 mu. the artificially cultivated yew seedlings have long been lulled into forests and have been highly praised by the relevant departments of the United Nations and the leaders of the domestic forestry department. It is expected that Hongdou Group will start production of paclitaxel raw materials and injections this year, with an annual output of 6 million paclitaxel injections. The project has passed the acceptance of the State Drug Administration and the provincial and municipal drug administrations. In addition, my country's northeastern forest areas and some mountainous areas in Yunnan and Guizhou provinces are also vigorously developing fast-growing yew forest cultivation techniques. In the next 3 to 5 years, these places are expected to form new yew forest areas, thereby providing valuable products for the country New sources of paclitaxel raw materials. Hunan, Hubei and other southern mountainous provinces are also building yew cultivation bases as one of the measures to help local farmers get rid of poverty and become rich. According to this development trend, the annual output of paclitaxel raw materials in China is expected to exceed 100kg in the next few years, thus becoming the world's major producer of paclitaxel raw materials and preparations. |
| precautions for injection | 1. hematological toxicity: as the main factor limiting the increase of dose, G-CSF should be used to assist when white blood cells are lower than 1500/mm3, and blood components should be transfused when platelets are lower than 30,000/mm3. 2. Allergic reaction: In addition to pretreatment, if there are only mild symptoms such as flushing, skin reaction, slightly faster heart rate and slightly lower blood pressure, it is not necessary to stop the drug, and the drip speed can be slowed down. However, if there are serious reactions such as low blood pressure, angioedema, dyspnea, and systemic measles, the drug should be stopped and given appropriate treatment. Patients with severe allergies should not be treated with paclitaxel again next time. 3. Nervous system: The most common is numbness of fingers and toes. About 4% of patients, especially at high doses, may have obvious sensory and motor disorders and reduced tendon reflexes. There have been individual reports of grand epileptic seizures during instillation. 4. Cardiovascular: transient tachycardia and hypotension are more common and generally do not need to be treated. However, it should be closely observed in the first hour of instillation, except for patients with severe conduction block, it is not necessary to observe every hour. 5. Joints and muscles: About half of the patients will feel joint and muscle pain 2 to 3 days after medication, which is related to the dosage used. It usually recovers within a few days. Muscle pain will worsen in patients given G-CSF. 6. Hepatobiliary system: Since most of the paclitaxel is excreted from bile, patients with hepatobiliary diseases should be observed with caution. In thousands of cases, about 8% of patients had elevated bilirubin, 23% of patients had elevated alkaline phosphatase, and 18% had elevated aspartate aminotransferase. However, there is no data to show that paclitaxel has serious damage to liver function. 7. Other: Although gastrointestinal reactions are common but generally not serious, a few may have diarrhea and mucositis. Mild alopecia is also more common. |
| chemical properties | needle crystal or amorphous powder is precipitated from methanol. Melting point 213~216 ℃ (decomposition). [α]D20-49 ° (methanol). UV maximum absorption (methanol):227,273nm(ε 29800,1700). |
| use | broad-spectrum anti-tumor botanicals for ovarian cancer, breast cancer, etc. broad-spectrum anti-tumor botanical drugs, used to treat ovarian cancer, breast cancer and other diseases anti-tumor drugs. For the treatment of metastatic breast cancer and metastatic ovarian cancer. Broad-spectrum anti-tumor botanical drugs for the treatment of ovarian cancer, breast cancer and other diseases effective anti-tumor drugs; combined with the N-terminal region of β-tubulin, it promotes a highly stable microtubule formation, Resist depolymerization, prevent normal cell division and capture in the G2 / M phase of the cell cycle. An anti-microtubule drug that inhibits depolymerization by promoting tubulin polymerization, maintains tubulin stability, and inhibits cell mitosis. On human endothelial cells, IC50 is 0.1 pM. |
| production method | natural products separated and purified from yew bark, woody roots, leaves, twigs and seedlings, with the highest content in bark. The scientific name of Taxus in the Botany of China is Taxus, and the plant classification belongs to the gymnosperm subphylum, Pinus, Taxus, Taxus. Taxus family contains 5 genera and 23 species. There are 4 genera, 12 species and 1 variety in China, namely Tibetan Taxus wallichinanaZucc or Himalayan Taxus, Taxus yunnanensis, Taxus chinensis vat.Mairei and Taxus cuspidata. Distributed in Tibet, Yunnan, Guizhou, Sichuan, Guangxi, Guangdong, Hunan, Hubei, Jiangxi, Fujian, Zhejiang, Anhui, Henan, Shanxi, Shaanxi, Gansu and Jilin mountainous areas. Extracted from the bark or leaves of Taxus plants. Taxus bark or leaves are dried in the shade, ground, and extracted with 95% ethanol. The extract is concentrated to dry, and the remainder is mixed with water and dichloromethane. The organic layer is separated from the rest, and the water layer is extracted with dichloromethane many times. After the extract and the organic layer are combined, they are concentrated to dryness. The residue is dissolved in ethyl acetate-methanol (3:1), mixed with fresh diatomite, and then evaporated to remove the solvent under reduced pressure. The remaining powder was subjected to rapid column chromatography, first washed with hexane, and then eluted with dichloromethane. The latter is collected and the dichloromethane is steamed under reduced pressure. The residue is dissolved in ethyl acetate, and 70 sets (3-4 sets per set) of medium-pressure rapid chromatographic columns with silica gel are used for chromatography, and different ratios of hexane-acetone are used for elution. Collect the components containing the product and concentrate it. The residue is then purified by a medium-pressure rapid chromatographic column filled with silica gel, and eluted with different ratios of methanol-dichloromethane. Collect the components containing the product and concentrate them under reduced pressure. The residue is then separated by a preparative high performance liquid chromatography, and the obtained product is recrystallized twice with aqueous methanol to obtain pure paclitaxel. Yield: bark 0.028%, leaves 0.0088%. Yield: bark 93.3%, leaves 88%. Melting point 212~214 ℃,[α]D20-49 (1%, chloroform). The stem bark of Taxus chinensis was crushed, dried in the shade, soaked in 1% citric acid aqueous solution for 24 hours and then began to percolate. The percolate is extracted with dichloromethane, the extract is dried, concentrated, and vacuum drained. Second column chromatography was performed with silica gel, and the collected effluent contained paclitaxel and harringtonine. Based on their different side chain structures, dissolve them in carbon tetrachloride and add bromine, only brominate harringtonine, and then undergo silica gel column chromatography to obtain an effluent containing only paclitaxel. After treatment, the taxol is obtained with a 99.19% content, a yield of 4.5 × 10-5, and a recovery rate of 70%. |
| EPA chemical information | The information is: ofmpub.epa.gov provides (external link) |
| toxic substance data | The information is: pubchem.ncbi.nlm.nih.gov Provide (external link) |
Last Update：2024-04-09 19:05:03
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